Template:Imperial/09/Overview

We are growing the cells to a desired OD of 0.7 in order to ensure that there are enough cells to produce a wanted amount of proteins

The first module is induced with IPTG and triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest.

This second module is where the bacterium, after having produced the peptide of interest, produces colanic acid thus creating a protecting layer around itself to shelter it from the acidity of the stomach.

Module 3 occurs after encapsulation of the bacterium containing the produced peptide of interest. This module has the purpose of making the bacterium non viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The bacterium is thus unable to produce any proteins and therefore unable to survive. It leaves behind an encapsulated bag filled with our proteins of interest to then be delivered to the gut.

Chemoinduction content

Autoinduction content

Thermoinduction content

Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.