Duke University/29 June 2009

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June 29, 2009

  • E: Restriction digests, Gel to check digests 
Digest 1
NdeI (FastDigest) 1 ul
SpeI (NEB) 1 ul
10x FastDigest buffer 2 ul
plasmid 1 ul (~100 ng)
H2O 15 ul
20 ul
Band Sizes/Lengths: 5 kb, 3 kb, 460 bp, (80bp)

Digest 2 (Did not do)
NdeI (FastDigest) 2 ul
10x FastDigest buffer 2 ul
plasmid 1 ul (~100 ng)
H2O 15 ul
20 ul
Band Sizes/Lengths: 5.4 kb, 3 kb

Digest 3
BamHI (FastDigest) 1 ul
XhoI (FastDigest) 1 ul
10x FastDigest buffer 2 ul
plasmid 1 ul (~100 ng)
H2O 15 ul
20 ul
Band Sizes/Lengths: 6.6 kb, 1.8 kb

Digest 4
BamHI (FastDigest) 2 ul
10x FastDigest buffer 2 ul
plasmid 1 ul (~100 ng)
H2O 15 ul
20 ul
Band Size/Length: 8.4 kb
  • R: Correct bands and sizes for plasmid 2 and digests 1, 3, and 4, correct band and size for plasmid 4
  • C: Do more tests on plasmid 2 such as sequencing to determine if plasmid is carryover, Try different primers
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