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Team:IPN-UNAM-Mexico/Notebook
From 2009.igem.org
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As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.
Protocols
July
August
September
October
Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:
| Number | Biobrick | Restriction |
|---|---|---|
| 2 | BBa_R0079 | S & P |
| 3 | BBa_F1610 | X & P |
| 4 | BBa_K091146 | S & P |
| 5 | BBa_K093005 | X & |
| 6 | BBa_K081016 | X & P |
| 7 | BBa_EC840 | X & P |
| 8 | BBa_K081009 | X & P |
| 9 | BBa_R0051 | S &P |
| 10 | BBa_J06800 | X & P |
| 11 | BBa_Q04121 | X & P |
| 12 | BBa_B0034 | S & P |
| 13 | BBa_C0079 | E & S |
| 14 | BBa_C0179 | E & S |
| 15 | BBa_B0015 | E & X |
| 16 | BBa_K081018 | E & S |
| 17 | BBa_K116640 | X & P |
| 23 | BBa_S03154 | X & P |
| 24 | BBa_k145201 | E & S |
| Ligation | Nickname | Biobrick |
|---|---|---|
| 4-23 | Ciencias I | BBa_K226005 |
| 13-15 | Ciencias II | BBa_K266002 |
| Ciencias I-7 | Ciencias III | BBa_K266006 |
| 24-17 | Ciencias IV | BBa_K266001 |
| 2-3 | Ciencias V | BBa_K266000 |
| J231000-11 | Ciencias VI | BBa_K266008 |
| Amplicon - Ciencias II | Ciencias VII | BBa_K266003 |
| Ciencias VI - 12 | Ciencias VIII | BBa_K266009 |
| Ciencias III - 19 | Ciencias IX | BBa_K266006 |
| J23100 – Ciencias VII | Ciencias X | BBa_K266004 |
| Ciencias III - Ciencias V | Ciencias XI | BBa_K266007 |
| Ciencias X - Ciencias IV | Ciencias XII | BBa_K266010 |
