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Team:Tokyo Tech/Iron-oxidizing bacteria
From 2009.igem.org
| Main | Team | Terraforming | Experiments | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Tokyo_Tech Parts] | Safety |
Contents |
Achievement
- We cultured iron-oxidizing bacteria (Acidithiobacillus ferrooidans).
Introduciton ~Why do we need to use Iron-oxidizing bacteria for terraforming of Mars?~
Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters.
Iron-oxidizing bacteria is autotrophic bacteria.
Iron-oxidizing bacteria gets energy by oxidizing Fe(Ⅱ).
To grow on Mars, iron-oxidizing bacteria require only energy injection of us because of composition of the Martian surface, crust and atmosphere. (リンク)
We propose culturing iron-oxidizing bacteria on Mars and producing organic matters.
Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli).
Material and method
Iron-oxidizing bacteria
Iron-oxidizing bacterium is a kind of autotrophic bacteria.
Iron-oxidizing bacteria reduce NAD+ to NADH by oxidizing Fe(Ⅱ).
Iron-oxidizing bacteria grow with dissolved CO2 as carbon source, not organic matters.
In laboratory, iron-oxidizing bacteria are cultured in 9K medium which contains Fe(Ⅱ) and no organic matters.
Preparation of 9K medium (for iron-oxidizing bacteria)
We Prepared following A and B solution.
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We sterilized A solution by autoclaving.
We sterilizde B solution by using filter.
We Mixed A and B solution before use.
Cultivation of Acidithiobacillus ferrooxidans
Result
Discussion
Reference
MEDIA FOORMULATIONS http://wdcm.nig.ac.jp/catalogue/ncim/document/Ncim_media.pdf
