Team:Kyoto/CiC/Notebook/0921-0925

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • TIM23complex signal::EGFP generator
    • miniprep PEGFP-N3 VECTOR (EGFP generator)
    • phosphorylation signal sequence primer

Results

  • 消えた

Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • TIM23complex signal::EGFP generator
    • PEGFP-N3 VECTOR (EGFP generator)

Results

  • 消えた

Wednesday, 23 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

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