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Team:BCCS-Bristol/BSim/Case studies/Magnetotaxis
From 2009.igem.org
Magnetotaxis - an agent based approach
The videos illustrate the differences between the use of magnetotaxis and chemotaxis as a method of controlling the motion of the bacteria. The first video shows magnetotactic bacteria influenced by a magnetic field strength of 7.53x10^(-6) Tesla. The field direction controls the alignment of the bacteria, but not the speed that they travel at. The higher the field direction, the stronger the alignment of the bacteria with the field direction. The second video acts as a comparison against the magnetotactic control, using chemotaxis. The bacteria detect a change in the chemical concentration, and move towards the higher concentration, i.e. up the chemical gradient.
Experiment: Effect of magnetic field strength on average velocity of bacteria
Several experiments were carried out on the bacteria. The first was to look at the effect of varying the magnetic field strength on the average velocity of the bacteria. The graph (Figure 1) below shows the result of this. As can be seen, there is a maximum average velocity of the bacteria after which increasing the magnetic field strength has no further effect on the magnetic dipole moment of the bacteria.
Experiment: Magnetotaxis versus chemotaxis
The next experiment was to compare the magnetotactic bacteria with the chemotactic effects on bacteria.
Using a chemical concentration of aspartate, the average velocity of the bacteria is not affected by different concentrations, as the bacteria will only detact a change, not by how much it has changed. Running this simulation for 100 bacteria, it was found that the average velocity of the bacteria was 76.30 microns/second.
In order to run a simulation with the same average velocity of magnetotactic bacteria instead, the magnetic field strength must be equal to 7.53x10^(-6) Tesla. The videos above show these two particular comparisons, i.e. the average velocity of the bacteria in the two videos are the same.
Additionally, the magnetic field strength can still be increased further, thereby increasing the average velocity of magnetotactic bacteria higher than that of chemotactic bacteria.
Finally, the ability to control the positions of the bacteria was compared for the two cases. The plots below (Figure 2) show the locations over time of individual bacteria, with magnetotactic bacteria on the left and chemotactic bacteria on the right. Clearly, while the two methods have the same average velocity, the deviation of the magnetotactic bacteria is much smaller. This means that it is easier to predict the location of a particular bacteria and so they are more easily controllable.
