Team:BIOTEC Dresden/Notebook SilverNano

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Silver Nano Particles

Strategy

Unique peptides have been found capable of nucleating metal atoms, spurred by an interest in biomineralization by the prospect of nanoscale building [1]. We use a twelve amino-acid long silver binding peptide that has been identified by combinatorial screening [2,3] to nucleate silver nanoparticles. The peptide is tagged with GFP so that it can be monitored along the flow chamber. Several protocols to bio-synthesize silver nanoparticles were tried in parallel, and absorbance of various samples were measured using spectrophotometry.



Methods

Here the basic ideas are introduced.


Results

Lab notes and obtained results.

[1] Lee, S., et al., Science, 296, 892-895, 2002
[2] Naik, R., et al., Nat. Materials, 1, 169-172, 2002
[3] Naik, R., et al., Nanobiophot. Biomed. Appl., 5331, 1605-7422, 2004


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