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Team:IPN-UNAM-Mexico/Notebook/October
From 2009.igem.org
October
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05-October-2009
We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.
06-October-2009
The digest L2-3(BBa_K266000) doesn´t work, we have to repeat it. Left 17 hours digest.
07-October-2009
We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone. Left 17 hours ligation overnight.
08-October-2009
Transformation by head shock, plate and left overnight in 37ºC.
09-October-2009
The transformation was successful we get five colonies.
12-October-2009
Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.
13-October-2009
We have culture and prepare midi prep.
15-October-2009
Biobricks sent to the registry and final ligation in progress.
