Team:Waterloo/Notebook/Protocols/Inoculation

From 2009.igem.org

Revision as of 02:59, 22 October 2009 by Timlin737 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Inoculation Protocol

Materials
DNA Samples
Ampicillin (2µL/sample)
Kanamycin (2.5µL/sample)
Liquid LB Media (1 tube/sample)

Instructions
1. Label all tubes.
2. Do the following in the flow hood:
- Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR.
- Dip the sterile stick into the DNA and into the corresponding media tube.
3. Incubate at 37 degrees Celsius overnight on the agitator.

Retrieved from "http://2009.igem.org/Team:Waterloo/Notebook/Protocols/Inoculation"