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Virginia Commonwealth/10 July 2009
From 2009.igem.org
Contents |
Friday 10 July 2009
Results
Afton and Maria
- Overnight culture was successful
Trentay 13:13, 10 July 2009 (UTC)
Tasks
Afton and Maria
- Make stocks of parts J23106 and J06702
- Miniprep of parts J23106 and J06702
- Digestion of parts J23106 and J06702
- Electrophoresis test of parts J23106 and J06702
- May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
- Write out complete plan for experimentation
- Make plans to MaxiPrep
- Write out a plan to organize ideas
Trentay 13:13, 10 July 2009 (UTC)
Wetlab
Afton and Maria
- Dilution of overnight culture
- 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
- 2 dilutions were done for each test tube
- Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer
- 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
- 500mL of 30 percent Glycerol stock was made
- Cell stocks were made
- 10 vials of J06702 were put in the -80 degree Celsius freezer
- 5 vials of J23106 were put in the -80 degree Celsius freezer
- MiniPrep was done on parts: J23106 and J6702
- Amount of DNA was calculated with a Spectrophotometer
- Digestion was performed on MiniPrepped parts
- There are two digested J06702 PCR tubes
- Digests will be frozen until Monday
- Electrophoresis test will be held off until then
- Ligation as well
Trentay 20:35, 10 July 2009 (UTC)
