Team:Wash U/Calendar-Home/1 July 2009

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Wednesday July 1, 2009

-Miniprep #2 to obtain more biobrick DNA

-New gel with digested vs. undigested plasmids

-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow

-Started new overnight cultures of biobrick parts and plasmids

-Team meeting #4

Miniprep #2:

1) BBa_I15010 pSB2K3

2) BBa_E0240 pSB1A2

3) BBa_R0082 pSB1A2

4) plasmid pSB1T3

5) plasmid pSB1K3

6) BBa_B0034 pSB1A2

7) plasmid pSB1AT3

8) plasmid pSB1A3

9) plasmid pucHS27

10) plasmid pSB1AC3

11) BBa_B0015 pSB1AK3

12) plasmid pRKCBC3

  • 35 ng/ul
  • 45 ng/ul
  • ng/ul

62.5 ng/ul

  • 42 ng/ul
  • 28.5 ng/ul
  • 133.0 ng/ul
  • 62.5 ng/ul
  • 54.0 ng/ul
  • 64.5 ng/ul
  • 31.5 ng/ul
  • 45.5 ng/ul 
Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
*Measured again with EtOH wipe after each measurement 7-15


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