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Indiana/6 July 2009
From 2009.igem.org
Grow liquid culture to prepare for mini prep
Preparation of Media
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media:
50 mL LB
50 uL KAN
Amp media:
50 mL LB
50 uL amp
add 3 mL of media to a tube
transfer 1 colony into each tube
shake @ 37 C o/n
