Thursday 23 July 2009

Maria and Afton

All overnight cultures grew except one vial of NEB 10 beta seed stock
- there was a second vial of seed stock that did grow successfully
Transformations were successful


Name	Type	Plate	Purpose	Growth Observation
NEB 10 beta	seed stock	LB	make more seed stock	lawn
NEB 10 beta	electro comp.	LB	(+) Control/ make more stock	lawn
NEB 10 beta	electro comp.	LB	(+) Control shocked	lawn
DB 3.1	electro comp.	LB	(+) Control shocked	lawn
DB 3.1	electro comp.	LB	(+) Control/make more stock	lawn
NEB 10 beta	electro comp.	Cm	(-) Control	no growth
NEB 10 beta	electro comp.	Cm	J23100 w/ J06702	6 small colonies

Trentay 17:19, 23 July 2009 (UTC)

Maria and Afton

Make cryogenic stocks of all overnight cultures
Make stocks of parts I1352 (RFP) and I13522 (GFP)
Update notebook, documents
Pick colonies from transformation
- pick controls as well to make more seed and electrocompotent stock

Trentay 17:25, 23 July 2009 (UTC) Kevin and Adam

We need to PCR DNA padding onto the first nine synthesized promoters. When we ordered these oligo's we did not consider that we needed additional overhang from the BioBrick prefix and suffix in order for the restriction enzymes to work properly. Doing this PCR will correct that error. Bussingkm 20:56, 23 July 2009 (UTC)

Maria and Afton

Trentay 17:25, 23 July 2009 (UTC) Kevin and Adam

First we reconstitute the DNA. The rule of thumb is that you take 1/2 the amount of oligo's (nmol) of H2O in uL for ~100x DNA stock solution. Store in (-20C)
- A problem was encountered while reconstituting the DNA. For several of the sequences the amount of water described above was not sufficient to reconstitute the DNA. It was too viscus and could not be pipetted at all. We doubled the amount of water that was used and made note of the approximate concentration.

|}

Virginia Commonwealth/23 July 2009