Team:Osaka/Meeting

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2009/08/01

2009/08/02

Competent　cellの作り方

  1. あらかじめ菌を培養しておく。
  2. Single colonyを試験管にとり、5mlのLBを加える。
  3. 37度で一晩おく。（over night→o/n)
  4. 大量に菌が得られたら、40ml LBを入れた三角フラスコに菌にいれて37度で約２時間Incubate。
  5. 分光光度計でOD=0.3になるまでIncubate。
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