Template:Team:KULeuven/13 August 2009/VanillinProduction
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Revision as of 12:45, 14 August 2009 by JochemDeen (Talk | contribs)
- Plated ComT (+RBS) from the registry
- Growing overnight in 37C
- Plated Ech
- From newly made liquid culture
- Miniprep of Ech and Sam8
- Nanodrop results:
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
Ech | 72,7 | 1,94 | |
Sam8 | 66,2 | 2,00 |
- Restriction digest on Ech and Sam8
- Gelelectrophorese on Ech and Sam8
- New electrocompetent cells were used for the electroporation using the old ligations
- Lig A -> Sam5 + Sam8
- Lig C -> Ech + fcs
- Note: there was no spark
- Plating of the electroporated cells
- 200 μl per plate twice
- 600 μl left of A and C