August/21 August 2009

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transformation

to get new plasmid

⑤(RBS+rhlR+terminator+promoter(reg))
⑦(RBS+cinR)
⑧(RBS+luxI)

check the result of transformation

2-8G     4
2-21F    0    
2-6O     2
2-13N   <15
⑤      0

restriction and digestion

ⅰ パーツ⑨ Insert H2O      4 No.2 buffer  2 ①ー3   12 EcoRI   1 SpeⅠ