August/23 August 2009

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Revision as of 09:33, 29 September 2009 by Unocchi (Talk | contribs)

1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies. 

(ligation sample No.) (no. of colonies)
          9                    1
         10                   100~
         11                   100~

inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)


2.digestion and ligation

'8/22 digestion sample
↓
gel electrophoresis
gel cut
↓
purification by [QIAquick Nucleotide Removal Kit]
↓
ligation

ligation
DNA        44
10* buffer 5
ligation   1
total      50uL

↓
16°C 3~4hr
↓
transfromation


3.transfromation 

ligation sample
⑤⑫⑬⑭⑮
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L]
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