"
Template:Team:KULeuven/29 August 2009/VanillinProduction
From 2009.igem.org
- Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required(SAMS II and EF II)
| Part | DNA ng | volume µl |
|---|---|---|
| sam5 | 100 | 0,8 |
| sam8 | 126,4 | 2,7 |
| ech | 100 | 1 |
| fcs | 244 | 2,2 |
- The 2nd part was first put on a gel electrophoresis and purified before it was used for ligation
- Gel electrophoresis of restriction on sam5, sam8, fcs and ech
- sam5 and sam8 showed expected results
- fcs and ech had unexpected signals
- fcs showed only 1 result on gel, expected two
- ech showed 4 results in gel (all too short), expected one
- sam5 and sam8 were purified and ligated (SAMS I)
| Part | DNA conc ng/µl | 260/280 |
|---|---|---|
| sam5 | 6,6 | 1,98 |
| sam8 | 4,1 | 2,88 |
- ech and fcs were cut again and put overnight (over the weekend...) in 16°C
