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Team:KULeuven/Project/Details
From 2009.igem.org
Contents |
Blue light receptor
The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. We designed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238002 BBa_K238002] part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238000 BBa_K238000] part was added to the registry.
Vanillin receptor
Vanillin production
Key antikey
