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Template:Team:KULeuven/12 August 2009/VanillinReceptor
From 2009.igem.org
- B,G and R were stored for further procedure. A failed again: there was no visible growth. This second time there was no problem with the competent cells because we used a commercial product instead of a self made. We assumed there was a problem with adding the polyA-tails to the PCR product because, when redoing the gelelectrophoresis, there were no errors with the PCR product.
- We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene was inserted correctly.
- PCR was performed for W,X,Y and Z to isolate the genes.
