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Template:Team:KULeuven/10 September 2009/BlueLightReceptor
From 2009.igem.org
- Since the results from our light experiments keep failing, some new ideas were computed. pSB1A2 is the backbone for our construct but since this is a high copy number plasmid perhaps the ratio of promotor to repressor is unequal (not enough repressor). A new enting in LC was done with ligX. These were grown for a few hours at 37°C and then put in 16°C room overnight so that more repressor can be made (its promotor is temperature-sensitive).
- Ligation from 09/08 was electroporated in competent cells.
- Miniprep of LigX, restriction digest and gel electrophoresis. The signals were good so a motherplate for ligX was made. Also, the vector pSB1A2 was extracted from the gel (39,1 ng/µl).
Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigX1(1) | 110,9 | 1,97 | |
| LigX1(2) | 107,3 | 1,95 | |
| LigX1(3) | 97,1 | 1,98 | |
| LigX1(4) | 106,9 | 1,97 | |
| LigX2(1) | 123,8 | 1,99 | |
| LigX2(2) | 100,9 | 1,99 | |
| LigX2(3) | 111 | 1,95 | |
| LigX2(4) | 108 | 1,97 |
