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From 2009.igem.org

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(6_September_2009 <|>8_September_2009)

Measurement of Receiver's Responce by AHL concentration (1)

• Making AHL water solution

We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.

• Making plates

Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.

• prior culture

We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .

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