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Team:Illinois/SgrS
From 2009.igem.org
SgrS Target-GFP Fusion
People in Group: Francis Lee, Harsh Shah, Dave Korenchan
Purpose: We want to fuse the SgrS target sequence from the PtsG gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the SgrS gene. We expect to see translational repression of GFP by the small RNA SgrS.
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 15
PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers.
June 16
We ran a gel on our PCR reactions, and the PCRs were successful!
