Team:NCTU Formosa/Project/Introduction

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- 4

Contact
- 6

Introduction

Bacterial referee with the adjustable timer and counter functions

Our team constructed a controllable synthetic genetic circuit in Escherichia coli which has timer and counter functions. The circuit works as an OR gate to integrate temporal and environmental signals.

The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After E.coli consume the added lactose, the RFP will be translated to remind us that time’s up.

The counter function can detect the bacteria population with LuxI/LuxR device; Moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.

Home	The Team	The Project	Parts Submitted to the Registry	Modelling	e-Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Find more information on how to use the calendar feature by going to the general calendar page.