Team:UNICAMP-Brazil/Notebooks/September 27

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YeastGuard

Dephosphorylation - CIAP test

  • The E.coli didn´t grow in any of the plates. =[ We think that we didn´t plate an enough volume of bacteria. So we decided to replate a larger volume. We hope to find colonies tomorrow!


Raíssa and Taís

ColiGuard

Inoculation of Yesterday's Ressuspended and Transformed Biobricks

  • We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them in liquid LB-AMP media.
  • Those inocula were grew for an O/N period at 37ºC (250 rpm).

Marcelo