The E.coli didn´t grow in any of the plates. =[ We think that we didn´t plate an enough volume of bacteria. So we decided to replate a larger volume. We hope to find colonies tomorrow!
Raíssa and Taís
ColiGuard
Inoculation of Yesterday's Ressuspended and Transformed Biobricks
We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them in liquid LB-AMP media.
Those inocula were grew for an O/N period at 37ºC (250 rpm).