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Imperial College London/Notebook/30 September 2009
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Nuri: -Did lacI/lactose diagram - Will read papers and continue CSS tutorial
Testing team
Harvard GFP
- Problems:
- Fluorescence was saturated for our positive control
- Conclusions:
- Fluorescence for the shifted sample (SS) shows increase of fluorescence overtime indicating that the temperature sensitive promoter (pLambda cI) is activated when shifted to 37°C
- However we could not determined whether the shifted sample reached the fluorescence level of the positive control as fluorescence readings were saturated on the fluorometer.
- The non shifted sample (kept at 28°C) was constant at fluorescence levels similar to the blanks. This suggests that the promoter repression is tight at 28°C.
- Blanks were constant
