Team:Johns Hopkins-BAG/A New Standard

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Building Blocks: a revolution in Large DNA/Genome assembly

The Johns Hopkins University Build a Genome team has proposed a new standard for the construction and assembly of large DNA. The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC10, RFC11,RFC12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly

This Building Block standard can be either:

A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part.