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Team:UC Davis/Adding secretion
From 2009.igem.org
Adding Secretion:
General
model for secretion system:
Secretion Models:
Click on a specific model for more information:
Advantages
of having several models:
The purpose
of the secretion system is to introduce a method
of excreting target proteins we wish to synthesize in the marvelous
host: E.coli
HB101. Through this approach of being able to secrete specific
target
proteins, we hope to serve as a chassis to the rest of the biological
world.
In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).
In our secretion system, we are using two genes with different sizes as our target secretion genes, GFP being short in length and Luciferase being comparably long would test our secretion system and its ability to secrete small and large proteins.
Testing multiple different combinations; Signal Sequence alone, Signal Sequence plus ompA/INPNC and ompA/INPNC alone, would help us find the best combination for our secretion system based on their secretion ability and rank them from strongest to weakest in strength (in this specific system).
Secretion Models:
Click on a specific model for more information:
There
is wide range of genes present in our environment. Therefore, it is
important
to measure our secretion system’s ability to secrete genes of various
sizes.
Molecular data on some proteins
|
|
Molecular |
Number
of residues |
Number
of polypeptide |
|
Cytochrome
c (human ) |
13,000 |
104 |
|
|
Ribonuclease
A(bovine pancreas) |
|
124 |
|
|
Serum
albumin (human) |
|
|
|
(Page
128, third edition “Lehninger
Principles of Biochemistry” by David L. Nelson and Michael M. Cox)
