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Team:Paris/DryLab
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iGEM > Paris > Home > DryLab > Main
Main
Problems
Our Message in a Bubble project is fully integrated inside a reflection on communication between bacterias. Therefore, the modeling studies aimed at answering 3 major problematics directly linked to the improvement of messengers and messages efficiency :
- How can we get a synchronisation between the maximum concentration of proteins encapsulated and the maximum quantity of vesicules sent by time units thus improving the quality of signal sent ?
- Can we make vesicules only by overexpressing soluble parts of membrane proteins ?
- How can we optimize the quality of the reception i.e getting a robust response with a limited number of messengers sent ?
Results
The synchronisation between protein creation and vesicules creation was acheived by the use of a transcriptional cascade inside the genetic network thus leading the a delay between the maximum concentration of proteins and of the maximum creation of vesicules :
SCHEMA
Indeed, the over expression of soluble parts.....
Pierre
, stochastic simulations revealed two possible problems reducing the robustness of our reception system :
- when the amount of messengers received is too weak, the activation does not always occur
- even when it occurs, the activation time can vary
