Team:Wash U/Biological Parts

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Parts

Component Part/Accession # Base Pairs Plasmid Resistance Well
RBS BBa_B0034 12 pSB1A2 A plate 1, 2M
Red Light Sensor BBa_I15010 2,238 pSB2K3 K separate
OmpR (E. coli) NP_417864.1 720 pSB1T3 T separate
Terminator BBa_B0015 129 pSB1AK3 A and K plate 1, 23L
OmpR + Terminator N/A calculate pany-amp A N/A
OmpC promoter BBa_R0082 108 pSB1A2 A plate 1, 16K
puc B/A N/A 375 pSB1A3 N/A







Component Part/Accession# Base Pairs Notes Plasmid Resistance Well
RBS 12 pSB1A2 A plate 1, 2M
Light Sensor Light Sensor 2,238 pSB2K3 K separate
OmpR (E. Coli) NP_417864.1 720 3' --> 5' pSB1T3 T separate
Terminator 129 pSB1AK3 AK plate 1, 23L
OmpR + terminator part bp pany-amp A
OmpC Promoter 108 pSB1A2 A plate 1, 16K
LH2 Output pucB/A R. Sphaeroides 375 (-40 of B to
stop of A)
pSB1A3 A separate
A YP_353390 # separate
B YP_353391 #
OmpC promoter+BA nopart number bp notes pany-kana K not on plate
PCB prod Light Resp Sys 4,333 *Source of
Hol/PcyA
plasmid A separate
TetR repressible 74 notes pSB1A2 A plate 1, 13B
GFP Marker Green
Flourescence
976 notes pSB1A2 A plate 1, 12M

Characterization