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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Tokyo_Tech Parts]

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Contents 1 Introduction 2 What’s darker colored E.coli? 3 Why do we need darker colored E.coli? 3.1 Increase Mars temperature 3.2 Get the heat energy 3.3 Protect DNA 4 How to make darker colored E.coli 4.1 Utilize Melanin production pathway 5 Materials and Methods 5.1 Constructed Parts 5.2 Assay 6 Discussion

Introduction

One of the famous black pigment is a Melanin which was utilized in a number of organism. Previously. It’s noted that the body color of E.coli was changed from white to black by a large amount of Melanin. Melanin was made by Tyrosinase (EC 1.14.18.1)(melA). Tyrosinase catalyze the first step of Melanin production pathway to make Dopaquinone. After production of Dopaquinone, Melanin is generated by nonenzymatical chain reaction. So, Overexprssion of tyrosinase in E.coli cause a large amount of Melanin. We considered these mechanism enable us to control the Mars temperature, get the heat energy that help to grow bacteria and, protect DNA from UV on the mars surface. Because of Melanin has a high efficiency heat absorption and absorb Ultra Violet. Therefore, we build a Tyrosinase into Bio-Brick parts and,

What’s darker colored E.coli?

Why do we need darker colored E.coli?

Increase Mars temperature

Get the heat energy

Protect DNA

How to make darker colored E.coli

Utilize Melanin production pathway

Materials and Methods

Constructed Parts

Assay

Discussion

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