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Story of a T-REX

(Trans-Repressor of Expression)

The main goal of our project is the realization of a device for post-transcriptional regulation of gene expression in Escherichia coli, in order to switch protein production of a gene of interest.



The Parts

To achieve this aim we designed, using BASER tool, two complementary 50bp non-coding sequences:

• CIS-repressing, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site (RBS):

5' Prefix - AACACAAACTATCACTTTAACAACACATTACATATACATTAAAATATTACAAAGAGGAGAAA - 3' Suffix

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