Team:Todai-Tokyo/Protocols/Infusion

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Infusion Description

Infusion is a method to combine DNA fragments using a combination of PCR and homologous recombination. It can be used as an alternative to ligation with the advantage that it does not require restriction enzyme digests, although specific primers are required for the reaction.

[http://bioinfo.clontech.com/infusion/ Clonetech page on In-Fusion]

Infusion Protocol

  1. Mix the purified PCR insert(50-200ng) and linearized vector(100-400ng)
  2. Add 1
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