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Team:Wash U/Calendar-Home/25 June 2009
From 2009.igem.org
Wednesday June 24, 2009
-Restreaked sphaeroides plates and plated from liquid cultures. We are working on getting rid of contamination on the earlier plates and in cultures.
-Waiting on supplies from Sigma Aldrich!
-Working on our webpage.... We have viewers from 4 continents!
-Minipreps of biobrick plasmid DNA (cultures from Th. 6/18)
-Overnight PCR with miniprepped DNA, to check for inserts
DNA nanospec concentrations (ng/microliter):
1) 22.2
2) 51.4
3) 135
4) 75.9
5) 81.4
6) 31.1
7) 49.6
8) 35.9
9) 62.9
10) 52.2
11) 134
12) 45.5
PCR (1) labels
PCR, Miniprep, Part #, Conc(ng/microliter), Plasmid, Plate
(A), 1, BBa_I15010, 22.2, pSB2K3, LBK50
(B), 2, BBa_R0082, 51.4, pSB1A2, LBA50
(C), 4, BBa_B0015, 75.9, pSB1AK3, LBA50
(D), 5, BBa_J04450, 81.4, pSB1K3, LBK50
(E), 7, BBa_B0034, 49.6, pSB1A2, LBA50
(F), 9, BBa_E0240, 62.9, pSB1A2, LBA50
(G), 10, BBa_J13002, 52.2, pSB1A2, LBA50
PCR Reactions to check Biobricks in Plasmids AccuTaq LA
5 uL of 10x Buffer 40 uL 2.5 uL of 10mM dNTP mix 20 uL 5 uL of template DNA (about 40 ng/uL) 1 uL DMSO 8 uL .5 uL 100X Primer F (Biobrick F) 4 uL .5 uL 100X Primer R (Biobrick R) 4 uL .5 uL Accutaq Enzyme 4 uL 35 uL H20 280 uL
50 uL
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