Team:Illinois/MicC
From 2009.igem.org
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MicC Target-GFP Fusion
People in Group: Phil Bell, Sonam Tanna, Davel Patel
Purpose: We want to fuse the MicC target sequence from the OmpC gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicC gene. We expect to see translational repression of GFP by the small RNA MicC.
Protocol(s) Used: (links to protocols page)
Recipe(s) Used:
Primers Used:
June 15
PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product.
June 16
Another PCR of the micC gene was done. Results should be analyzed tomorrow.
June 17
We performed a restriction enzyme digest of our small RNA and its target sequence. We then ran a gel to purify the DNA.