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Team:TorontoMaRSDiscovery/Notebook/April
From 2009.igem.org
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April 27, 2009
- Received from Rosa (SPiT):
- TM0785
- Plasmid containing encapsulin
- Recommend transfect into bacteria and re-sequence
- See email note regarding sequence error
- 0.5 microliters TMG DNA 100 microgram/microliter
- Use 0.4 microliter for 50 microliter PCR reaction =August 1, 2009=
- TM0785
- Overnight encapsulin cultures 1 and 6 (randomly selected) and the negative control were miniprepped
- The rest of the encapsulin cultures were stocked with 20% glycerol
- 6 digestions were done: Enc1, Enc6, Enc -ve, BB5, BB7, and C
- 16ul of BB5 plasmid was used
- 500ng of plasmid were used for the others
- The digestions were run on a 1.3% agarose gel in TAE (from here on, unless otherwise specified, all gels were 1.3% agarose)
- BB5 was confirmed and all other parts were correct as well
- Overnight ligation of 7+Enc in the PCR machine
