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Parts
Measurement Kit
Main Article: Measurement
| Name | Part type | Registry link
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| pSMB_MEASURE: Promoter Measurement plasmid (mammalian) | Plasmid backbone | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
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| pSMB_REFERENCE | Plasmid | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]
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Core promoters
Main Article: Core promoters
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.
| Name | Part type | Registry link
|
| JeT | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
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| JeT proximal-CMV core | Regulatory | [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]
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Promoters created by RA-PCR
Main article: Synthetic promoters
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
| Name | Part type | Registry link
|
| Constitutive promoter; 0.4 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
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| Constitutive promoter; 1 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
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| Constitutive promoter; 2 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
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| Constitutive promoter; 2.5 REU | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
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| NF-κB regulated promoter | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
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| pPARγ regulated promoter 1 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
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| pPARγ regulated promoter 2 | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]
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HEARTBEAT Predicted promoters
coming soon...
Natural promoters
Main article: Natural promoters
| Name | Part type | Registry link
|
| LDL receptor promoter (sterol regulated) | Regulatory | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
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| HMG-CoA synthase promoter (sterol regulated) | Regulatory | [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
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| c-Jun promoter (growth factor regulated) | Regulatory | [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]
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Targeting Signals
Main article: Outlook and Summary
Protein domains for targeting to sub-cellular compartments.
| Name | Part type | Registry link
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| Plasma membrane targeting (GPI anchor) | Protein domain | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
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| Plasma membrane targeting (myrpalm) | Protein domain | [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
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| ER membrane targeting (C-terminal) | Protein domain | [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
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| ER membrane targeting (N-terminal) | Protein domain | [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
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| mCherry-myrpalm-polyA | Composite | [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
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| GFP-polyA | Composite | [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
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