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Team:Chiba/Project/Signaling-system
From 2009.igem.org
Signaling System
- なんじゃこりゃーなおします。--Yoshimi 13:22, 20 October 2009 (UTC)
- sender使わないからいらないのでは？というか，cell-cell comunicationは使ってなくてただのtranscription activatorとしてluxRを使っているだけ...という面がつよい...ので，luxRの転写活性化がどのようにおこるか，をメインにしてはいかが?--Maiko 13:43, 20 October 2009 (UTC)
- 次項のスクリーニングでsenderを使うので、ここでも図にSenderを入れておくべきだと考えます。--Yoshimi 14:10, 20 October 2009 (UTC)
- まあAHL合成用としてluxIが入った細胞は使うのだが，プロジェクト的にはcell-cell communicationは関係ないので，「センダー」「レシーバ」の概念をいれないほうが話の筋はとおる気が...？luxIは横でフツーに説明すればok。とおもいまいた。が，まあigem communityでluxR-luxIがわからない人はいないと思うので書いてもまあ問題はないかもしれません。 --Maiko 14:27, 20 October 2009 (UTC)
In this project, we use acylated homoserine lactones (AHLs), signaling
molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria.
Senders express LuxI or similar enzymes, which catalyze the production
of AHLs, under the control of a constitutive (Tet) promoter. Each cell
thus generates AHL more or less at a constant rate. AHL can freely
permeate cell membranes and are detected by neighboring cells.
Receivers constitutively express LuxR proteins (or a similar
ortholog), the protein that detects AHL concentrations. When AHLs bind
LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The
threshold [AHL] at which switching occurs is determined by the
affinity of AHL for the particulr LuxR ortholog.
about quorum sensing)
