Team:Kyoto/CiC/Notebook/week2

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Contents

1 Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide
- 1.1 Monday, September 16
  - 1.1.1 To Do on 24 July
  - 1.1.2 Results
    - 1.1.2.1 Miniprep
    - 1.1.2.2 Digestion
- 1.2 Tuseday, September 11
  - 1.2.1 To Do on 24 July
  - 1.2.2 Results
    - 1.2.2.1 Ligation
    - 1.2.2.2 Electrophoresis

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 16

To Do on 24 July

Miniprep (HIV TAT LALAA peptide_terminator)
Restriction Enzyme Digestion

Results

Miniprep

HIV TAT LALAAA_terminator was minipreped.

Digestion

Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.

Sample list
NO.	Sample Name	Enzyme
1	1-N-23	Spe1, PST1
2	HIVTAT	Xba1, PST1

Tuseday, September 11

To Do on 24 July

Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
Transformation

Results

Ligation

Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

Sample list
NO.	Sample Name	Enzyme
1	3-O-2	Eco R1, PST1
2	3-A-4	Eco R1, PST1
3	3-O-2	Eco R1
4	3-A-4	Eco R1

Electrophoresis

Very clear bands were found. (See the gel image in right!)
The bands are listed below.

Parts and Plasmid length
	3-O-2	3-A-4
Parts length / bp	2837	487
Plasmid length / bp	3266	3266

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