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Duke University/18 June 2009
From 2009.igem.org
June 18, 2009
- E: Assembly PCR, Gels to check PCRs
2x Phusion mix 12.5 ul insert 7.14 ul H2O 5.36 ul 25 ul 98°C 30s 1x 98°C 10s 70-55°C 5s 72°C 30s 25x 72°C 2m 4°C infinity 1x Fragment # Name Size/Length (bp) Concentration (ng/ul) Mass (ng) Volume (ul) 1 pASKphaA 4215 41 194 4.73 2 phaB 896 68 41 0.60 3 Termcat2 1701 69 78 1.13 4 phaC 1872 126 86 0.68 ~400 7.14
Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
- R: Smear because of misformed gel, no band
- C: Change conditions
