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Safety
About: Safety in the Lab
1.Would any of our project ideas raise safety issues in terms
of:
-researcher safety,
-public safety, or
-environmental safety?
Standards for a Security Level 1 lab:
The lab where our iGEM team works, is a security level 1 lab
according to the Gentechnikgesetz (law for gene technology). This means
that the lab conforms to standards that our instructors introduced us
to in the beginning of the project in April 2009. After a safety
briefing for the group, we had a “safety-tour”
through the lab while
our advisor Kristian told us how to handle the different instruments
like centrifuges and Bunsen burners and showed us were to find the fire
extinguishers, safety showers and eye showers and explained the
handling. In the lab we store highly inflammable materials and gas
containers in a safety cabinet.
When we were working with hazardous chemicals or genetically modified
organisms we were wearing suitable protection gear such as lab coat,
safety glasses and gloves.
We disinfected our hands, the pipettes and the bench before and after
working with bacteria and phages. Most of the material we use in the
lab has to be sterile and it went through the autoclave before and
after using. All the bacteria waste was collected in autoclave bags and
autoclaved before delivered to the waste containers to prevent an
environmental pollution.
While working with toxic stuff or reagents that are harmful to health
we additionally used the hood as working station. When using liquid
nitrogen or working with UV-light we were wearing safety glasses and
gloves and handled our reagents with care.
2. Is there a local biosafety group, committee, or review
board at your institution?
In Germany we have by law a safety officer in each lab/working
group who controls their work and notes.
3. What does your local biosafety group think about your
project?
We made sure to follow all regulations described by the
Gentechnikgesetz (law for gene technology) including the
Gentechnik-Sicherheitsverordnung-GenTSV (gene technology
safety-regulation), the Gentechnik-Aufzeichnungsverordnung-GenTAufzV
(gene technology recording regulation) and the
Gentechnik-Verfahrensverordnung-GenTVfV (gene technology proceeding
regulation).
Thus we classified our work as safety-level one work.
4. Do any of the new BioBrick parts that you made this year
raise any safety issues?
