Team:BCCS-Bristol/Meetings

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BCCS-Bristol
iGEM 2009

Contents

Meetings

9th July 2009

Vesicles with a twist...part II

After a week's worth of digging through the literature we have managed to find possible non-toxic candidate proteins that pack within outer membrane vesicles produced by E.coli. Thus we have settled into implementing directed communication and delivery of proteins of interest between different cells/populations. This will possibly form the bigger part of the project and if time permits we will combine this with our magnetotaxis idea.

With respect to modelling we are interested in developing a useful tool for the community and research by possibly expanding the existing BSim software. For further information visit the modelling page of the wiki.

With respect to the lab we will pursuit the building of biobricks that will allow the implementation of proteins of interest into OMVs (outer membrane vesicles) as a first part to the project. Upon the succesful completion of this first task we want to implement some GRN for the control of responses of both production and/or message acceptance.

A copy of the presentation of this meeting can be found here.


2nd July 2009

Vesicles with a twist

We seem to have settled down to the idea that implementing magnetotaxis in vivo in E.coli will be infeasible within the given time-frame. Instead we have decided to move in the direction where we will try implementing the usage of vesicles in our project.

We are still thinking of perhaps implementing magnetotaxis combined with bacteria in an in vitro manner.

26th June 2009

Bacterial diagnosis

Ollie and Thanasis spent some time this week looking into whether we could use bacteria to detect and report on the state of an organism. It now seems we have now dropped the idea on the grounds it is too similar to projects attempted in previous years (NYMU, KULeuven) and would require licensing. However there was a suggestion that this idea could be taken in the direction of sequence based toggle switches to carry out a (more complicated) decision tree based diagnosis.

Magnetic bacteria

Petros prepared some slides on the biological feasibility of engineering E.coli to synthesize magnetosome (Copy of the presentation found here)

Links to the papers cited in the presentation can be found here:

  • [http://www.nature.com/nrmicro/journal/v2/n3/full/nrmicro842.html Magnetosome formation in prokaryotes]
  • [http://pubs.acs.org/doi/abs/10.1021/cr078258w Magnetotactic bacteria and magnetosomes]
  • [http://www.jbc.org/cgi/content/full/M106408200 A Magnetosome-specific GTPase from the Magnetic Bacterium Magnetospirillum magneticum AMB-1]
  • [http://aem.asm.org/cgi/content/abstract/67/10/4573 A large gene cluster encoding several magnetosome proteins is conserved in different species of magnetotactic bacteria]
  • [http://www.jbc.org/cgi/content/abstract/277/37/33559 The Escherichia coli Cytochrome c Maturation (Ccm) System Does Not Detectably Attach Heme to Single Cysteine Variants of an Apocytochrome c]
  • [http://aem.asm.org/cgi/content/abstract/75/12/3972 Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic sediments]
  • [http://www.ncbi.nlm.nih.gov/pubmed/18537832 Genetics and cell biology of magnetosome formation in magnetotactic bacteria]
  • [http://www.ncbi.nlm.nih.gov/pubmed/16303747 Complete Genome Sequence of the Facultative Anaerobic Magnetotactic Bacterium Magnetospirillum sp. strain AMB-1]


Steve and Emily looked into possible applications of magnetotaxis. These papers seem particularly interesting:

  • [http://link.aip.org/link/?APPLAB/89/233904/1 Controlled manipulation and actuation of micro-objects with magnetotactic bacteria]
  • [http://wiki.polymtl.ca/nano/images/0/00/C-2006-MTB-IARP-Sylvain.pdf Towards Autonomous Bacterial Microrobots]
  • [http://www.polymtl.ca/recherche/rc/en/professeurs/details.php?NoProf=122&showtab=PUB Other publications by Sylvain Martel]

Vesicles

Tom gave a presentation developing the idea of communication and self-assembly using vesicles.

18th June 2009

The shortlist was down to 7 projects and 3 of those made it to the next round as voted by the present audience. These are as follows (project title, (votes received)):

  • Magnetic Bacteria (10)
  • Bacterial Vesicles (6)
  • Non-Invasive Reporting/Switches & Sensors (6)
  • Genome Updates & version control (5)
  • Host/Parasite Symbiosis (3)
  • Bacterial Computation
  • RNA Editing

Thus the top three were chosen to review the literature further and assess the possibility of carrying each out in 3 months.

11th June 2009

Kick off meeting. Narrowed down ideas from ~15 to 7. See Team:BCCS-Bristol/Brainstorming.

2nd June 2009

Presentation of initial ideas at MCB2009.

Calendar

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