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Team:Warsaw/Calendar-Main/10 July 2009
From 2009.igem.org
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Team meeting
- presentations of work did by both groups during last week (given by Ania and Kuba)
- presentation of methods used by teams starting in previous editions of iGEM to kill bacteria :) (Kamil)
- presentation of different methods of targeting drugs to cancer cells (Marcin)
We've been also talking about some organization issues and we've decided to move our meetings - now they will take place every Thursady at 17:00, one week at the Faculty of Biology Building, another week in the room E of the lab on Pawinskiego Street.
Miecznikowa team: Creating devices to test promotors in E. coli strains (devices BBa_K177024 and BBa_K177025)
Franek
Task:
- Alkaline lysis of the plasmid containing BBa_B0024
- Setting cultures with BBa_R0010 and BBa_R0080
Methods:
- PlasmidMini set by A&A Biotechnology was used. 2 test tubes with 5ml LB and Ampicyline were first inoculated with colonies containing BBa_B0024 brick on pSB1A2 plasmid. The cultures were incubated for 6h at 37C.Alkaline lysis was performed on both cultures. The pellet from 5 ml of bacteria was used.
- 3 test tubes with 5ml LB and Ampicyline were inoculated with colonies containing BBa_R0010 brick on pSB1A2 plasmid. The cultures were left for over night incubation at 37C.
- 3 test tubes with 5ml LB and Ampicyline were inoculated with colonies containing BBa_R0080 brick on pSB1A2 plasmid. The cultures were left for over night incubation at 37C.
Results:
- Concentration of DNA sample was measured using NanoDrop ND-1000.
| DNA sample | DNA concentration in ng/ul |
|---|---|
| BBa_B0024 | 33.82 |
Notes:
- Plates with BBa_I0500 transformants were empty once again. This result, together with note in part description (http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I0500) suggest that this part is damaged.
Jarek
Task:
- Digestion of the parts C0051 and B0032
- Electrophoretic separation of digested parts
- Isolation of DNA samples from gel
- Ligation of part C0051 to the wector with B0032
Methods:
- DNA samples were digesteg with 1 ul of PstI/BcuI (B0032) or PstI/XbaI (C0051) in 1xTango buffer for 3 hours.
- After digestion they were separated on 0,8% agarose gel.
- Isolation of sample from gel was performed with A&A "Gel-out" kit.
- For ligation 4ul of ligase buffer and 2 ul of ligase were used.
Results:
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