Progress

To do:

Strain selection
- Odor-free E.coli available on partsregistry () [ordered]
Order iobricks for Vanillin detection [Done]
Primer selection for the promotor [Done]
- Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]
Biobricks for vanillin synthesis
- Send mail to University of Tuscia [Done]
- Order:
  - (sam8 (tyrosine-ammonia lyase) coding sequence) [on hold]
  - (sam5 (coumarate hydroxylase) coding sequence) [on hold]
  - (COMT gene with ribosome binding site) [Available in kit]

Extra info on Vanillin synthesis

[http://www.ncbi.nlm.nih.gov/pubmed/16232583 Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin. B C Okeke and V Venturi ,J Biosci Bioeng. 1999; 88(1): 103–106.]