Team:Paris/Protocols

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Protocols

iGEM paris 2007 protocols link :

content :

• Growing bacteria in liquid medium
• Preparing growth media
  • exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)
    

Solid M9 Minimum Medium
Preparation de gel d'agarose

• Chemical transformation
• Glycerol Stock
• Recombineering/Lambda red-mediated gene replacement
• Miniprep
• Fluorescent single cells visualisation
• Digestion reactions