Virginia Commonwealth/24 July 2009

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Contents

Monday 20 July 2009

Results

  • Results of transformation with pSB4C5 backbone in NEB 10 beta three days after transformation
    • Control (+) on LB NEB 10 beta (not shocked) had Lawn growth
    • Control (-) on Cm NEB 10 beta (not shocked) had No growth
Promoter Reporter Resistance/Plate Growth Observation
J23110 E0240 Cm 62 colonies
J23110 J06702 Cm 56 colonies
J23113 E0240 Cm 75 colonies
J23113 J06702 Cm 33 colonies
J23116 E0240 Cm 41 colonies
J23116 J06702 Cm 65 colonies
R0011 E0240 Cm hundreds of colonies
R0011 J06702 Cm 5 colonies
R0040 E0240 Cm 20 colonies
R0040 J06702 Cm 12 colonies
J23105 E0240 Cm 26 colonies
J23101 E0240 Cm 34 colonies

Trentay 14:17, 20 July 2009 (UTC)

Craig and Clay

  • The registry did confirm that the correct resistance for the limonene synthesis related parts is ampicillin. New parts will also be sent.

Tasks

Maria and Afton

  • Pick colonies for overnight culture from transformations
  • Ligate J23106 and J23101 with backbone pSB4C5 and RET E0240
  • Transform liagated parts into NEB 10 beta

Trentay 18:40, 20 July 2009 (UTC)

Wetlab

  • 1
  • 2
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