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Team:Todai-Tokyo/Notebook/bioclock
From 2009.igem.org
Plan
Aim: Create E.coli that show oscillatory gene expression pattern
Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.
- plate1,13B
- plate1,13L
- plate2,1H
2. Make a gene network that express oscillatory pattern, using these genes.
Cloning the parts
7/7
preculture of the Biobrick parts for Miniprep
7/8
Miniprep of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
failure
7/9
Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
Plan
Miniprep(7/29)
- P1.14L(araC)
- P1.7L(lacI)
- P1.4E(cI)
- P1.3D(ColE1)
- P1.9C(p15A)
- P1.9G(p15A)
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