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Minnesota/24 June 2009
From 2009.igem.org
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Since there are several problems with the first TNN model, we are deciding to focus on them one at a time. The first thing to work on is the leakiness problem. In order to deal with this issue, we are including a reaction that allows for the binding of the RNA polymerase to the operator site even if the site is occupied by a tetR. This will allow for some expression at an aTc concentration of 0, because at this point tetR is almost always bound to the tetO operator.
Here is the model we are working on:
| Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|
| RNAp + lacP + lacI4:lacO1 -> RNAp:lacP | 6.23E+05 | |
| RNAp + lacP + lacO1 ↔ RNAp:lacP | 1E+07 | 1 |
| RNA:lacP -> RNAp:lacP* | .01 | |
| RNAp:lacP* -> lacP + lacO1 + RNAp:DNAgfp | 30 | |
| RNAp:DNAgfp -> RNAp + gfp_mRNA | 30 | |
| gfp_mRNA + rib -> rib:gfp_mRNA | 100000 | |
| rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA | 33 | |
| rib:gfp_mRNA_1 -> rib + gfp | 33 | |
| tetR2 + aTc ↔ tetR2:aTc | 2E+09 | 4E-04 |
| tetR2:aTc + aTc ↔ tetR2:aTc2 | 1E+08 | 1E-03 |
| tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc | 1E+08 | 1 |
| tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E+05 |
| tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc | 1E+08 | 1E-03 |
| tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2 | 1E+08 | 1E-03 |
| tetR2 -> Ø | 2.89E-04 | |
| tetR2:aTc -> aTc | 2.89E-04 | |
| tetR2:aTc2 -> 2 aTc | 2.89E-04 | |
| tetR2 + nsDNA ↔ tetR2:nsDNA | 1000 | 3.2409 |
| tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA | 1000 | 3.2409 |
| tetR2:aTc:nsDNA -> aTc + nsDNA | 1.93E-04 | |
| tetR2:nsDNA -> nsDNA | 1.93E-04 | |
| Ø -> tetR2 | 1E-11 | |
| aTc_ext -> aTc | 3.3E-04 | |
| gfp_mRNA -> Ø | 1.16E-03 | |
| gfp -> Ø | 3.21E-05 | |
| RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 311000 | |
| RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 311000 | |
| RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 311000 |
