Virginia Commonwealth/31 July 2009

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Results

Maria and Afton

  • Overnight culture of I1352 grew, but no visible florescence
  • Transformation Results below
Name Plate Purpose Growth Observation
NEB 10 beta LB (+) Control shocked lawn
NEB 10 beta LB (+) Control lawn
NEB 10 beta AMP (-) Control no growth
NEB 10 beta CM (-) Control no growth
NEB 10 beta CM I1352 ligated to the backbone pSB4C5 no growth
NEB 10 beta AMP I1352 ligated to the backbone PSB4C5 hundreds of colonies; some expressing RFP
NEB 10 beta AMP I1352 ligated with itself hundreds of colonies; some expressing RFP

Trentay 15:25, 31 July 2009 (UTC)

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