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CAROL
Modelling Meeting
- No lab experiments performed today (have to re-consider methods) - no colonies were found from the transformation that occured yesterday morning.
- For the modelling meeting, the following points were discussed:
1. A better organization of our work is required. We have both characterization and simulation from the mathematical team, but the work seems unlinked and not organized. Will have a more organized report for next week.
2. Greater detail was explained to us by Thane Kubik regarding the biochemical details that are behind the signalling pathway.
3. We need to start thinking about how we are going to present our two modelling system that we have created.
4. We will meet again on thursday and Afshin will give us a better overview of membrane computing.
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CHINMOYEE
I'm Back
Today we had a really great and important modelling meeting. We looked at the biochemistry of the circuit and gained new insight on different concept concerning the circuits.
Iman showed us the visual representations he created .
A few pictures for the APEGGA article was taken and the final article was revised.
I revised the math modelling summary and sent it out to everyone. The simbiology simulations were also revised . New reactions were added to the old file.
Researched information on GFP and thought about how that individual circuit/ mutant circuit could be characterized .
Learned how to update on wiki.
New Jobs to do :
Prepare for the presentation on Friday : 12 min
Gauntlet article due Friday
Try to make an easier interface on simbiology ( eg . changing the initial concentration of ...)
Think about flow/content of Jambouree presentation :5 min content
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EMILY
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FAHD
Marketing for August 4th
Today, I put my energy towards marketing our project. I made preparations for our 2009 iGEM fundraising Bake Sale which will be held tomorrow (Wednesday August 5th 2009).
I also worked on some grant stream applications such as t=The Enbridge Pipeline Inc. Community Support Grants and also contacted some Oil & Gas companies. The following is the list of companies I contacted today:
1)Mackenzie Aborginal Corporation (MAC)
2) KMC Mining
3) Imperial Oil Resources.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Preparation for Plasmid switch of Pqrr4+I13500
Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight.
Overnight cultures of our reporter circuit
In order to further verify our reporter circuit (Pqrr4+B0034+K082003 Colony 9) using restriction digest, an overnight culture needs to be grown; thus 2 were grown and are to be shaken overnight at 37˚C
Modelling meeting
We had our first modelling dedicated meeting today, collaberating with the membrane computing team. We becan clearly outlining the differences and advantages/disadvantages of membrane computing and our methods of modelling.
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MANDY
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PATRICK
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PRIMA
Newsletters, sponsorship & Baking
Today I followed up with a few companies. Some companies need a few more days to reconsider. I also got hold of a few more company staff and emailed our sponsorship package out. I will follow up with them on Thursday August 6, 09. Cayman Chemicals declined our offer due to economic contraints. However, Sigma Aldrich has kindly donated $250.00 to iGEM Calgary. I emailed our July newsletter to all the companies that I was in-charge of and finished updating the sponsor's list on gmail. I also began working on Friday's team presentation. Aside from following up with sponsors, I called Hyatt and changed one of the triple rooms to a quad room. For tomorrow's bake sale, i confirmed the booking of the bake sale location with the main office.
I wrote up a short introduction to Nexen for the wiki as they are our sponsor of the month this month! Finally, I finished off the day at work with updating the daily notebook. Now i'm off to baking for tomorrow's bake sale.
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STEFAN
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VICKI
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